Detection and Assay Kits

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chemicals

Detection and Assay Kits

Cell Proliferation Kits
Coelenterazine Sampler Kit
Staining Kits

Cell Proliferation Kits

MTT Cell Proliferation Colorimetric Assay Kit

Coming soon!

Coelenterazine Sampler Kit

Storage: Store at -20°C
Protect from light
Purity: >98%

This kit contains 25µg each of nine coelenterazine analogs: coelenterazine, coelenterazine cp, coelenterazine f, coelenterazine fcp, coelenterazine h, coelenterazine hcp, coelenterazine i, coelenterazine ip, and
coelenterazine n.

Live & Dead Cell Staining Kit

Performs 300 assays. Kit components include:

2 vials (50 uL/vial) Calcein AM, 4mM
2 vials (150 uL/vial) Ethidium Homodimer-III (EthD-III), 2 mM

Live & Dead Cell Staining Kit provides a two-color fluorescence staining on live cells ( green ) and dead cells ( Red ) using two probes. Calcein AM stains live cells green while EthD-III stains dead cells red. These probes measure two recognized parameters of cell viability — intracellular esterase activity and plasma membrane integrity. The kit is suitable for use with fluorescence microscopes, fluorescence multi-well plate scanners and flow cytometers. The assay principles are general and applicable to most eukaryotic cell types, including adherent cells and certain tissues, but not to bacteria or yeast. This fluorescence-based method of assessing cell viability can be used in place of trypan blue exclusion, 51 Cr release and similar methods for determining cell viability and cytotoxicity. EthD-III (cat# 40050 and 40051) is a proprietary DNA stain and is a superior alternative to EthD-I offered by our competitor. EthD-III has higher DNA binding affinity and thus brighter fluorescence (40% brighter than Ethidium homodimer I).

Features:

Dual Detection
Detect live and dead cells in a cell population simultaneously.
Simple & Fast
30 min dye loading and measure without washing.
Economic
Perform viability and cytotoxicity assays at the same time.
Versatile
Analyzed by flow cytometers, fluorescence microscopes and fluorometric plate readers.