D-Luciferin is a natural compound isolated from fireflies and is a substrate for the enzyme luciferase. Chemiluminescence at 560 nm is generated when luciferin is oxidized by an ATP-dependent process, catalyzed by luciferase. Luciferase is encoded by the luc gene, which is widely used as a reporter gene in a variety of cells. Because of the intrinsic low background of chemiluminescence technique, detection of the luc gene expression can be made at very low level. In addition, luciferin/luciferase has been used to measure 10 -15 molar quantity of ATP. White solid soluble in DMSO and slightly soluble in water. |
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