D-Luciferin & Luciferase Assay Kits

Firefly & Renilla Luciferase Assay Kit (available in 100-assay and 1000-assay kits)

Kit Components:

• D-Luciferin
• Coelenterazine
• Passive Lysis Buffer
• Firefly Luciferase Assay Buffer
• Renilla Luciferase Assay Buffer
• Renilla Luciferase

Features:
Linearity and Sensitivity
Linear correlation between luciferase gene and light output for transfection using 0.001 ug to 1 ug DNA of either Firefly or Renilla luciferase reporter constructs.

Low Autoluminescence
Reduced autoluminesence background for Renilla luciferase and increased sensitivity.

Convenience
One assay kit for both luciferase assays.

Description:
Firefly and Renilla luciferases are widely used as reporter genes for studying gene regulation and function, and for pharmaceutical screening. Renilla Luciferase is often used in conjunction with Firefly Luciferase as a normalizing transfection control or for multiplex transcriptional reporter assays. As with many enzymes, Firefly luciferase and Renilla luciferase follow Michaelis-Menten kinetics and thus maximum light output is not achieved until substrates (above the Km) and co-factor are present in large excess. When assayed under these conditions, light emitted from the reaction is directly proportional to the number of luciferase enzyme molecules. MIP's Firefly & Renilla luciferase assay kit is designed for detection and quantification of Firefly and Renilla luciferase reporter enzymes from cultured cells in a simple, efficient and linear fashion.

Renilla Luciferase Assay Kits (available in 150-assay and 1000-assay kits)

Kit Components:

• Coelenterazine
• Renilla Luciferase Lysis Buffer
• Renilla Luciferase Assay Buffer
• Renilla Luciferase Assay Enhancer

Features:
Linearity - Linear correlation between luciferase gene and light output for transfection using 0.001 ug to 1 ug DNA of a Renilla luciferase reporter construct.

Low Autoluminescence & Sensitivity - Reduced autoluminesence background and increased sensitivity by using a proprietary substrate.

Convenience - Lysates can be used for performing Firefly luciferase assay.

Description: Renilla luciferase has been used as a reporter gene for studying gene regulation and function in vitro and in vivo. Recently, Renilla luciferase has been widely used in multiplex transcriptional reporter assays or as a normalizing transfection control for Firefly luciferase assay. Renilla luciferase, a monomeric 36,000 Dalton protein, catalyzes coelenterazine oxidation by oxygen to produce light. The enzyme does not require post-translational modification for its activity, and may function as a genetic reporter immediately following translation. Coelenterazine native is the natural substrate for Renilla luciferase. However, over a dozen of coelenterazine analogs have been synthesized, many of which are now commercially available from MIP. These coelenterazine analogs all function as substrates for Renilla luciferase with different properties in terms of emission wavelength, cell membrane permeability and quantum efficiency. Coelenterazine also emits light from enzyme-independent oxidation, a process known as autoluminescence. The autoluminescence is enhanced by superoxide anion and peroxynitrite in cells and tissues. MIP's Renilla Luciferase Assay Kit is designed to provide a simple and sensitive method of detecting Renilla luciferase. Through the use of a specially designed coelenterazine derivative and buffer formulation, this assay kit yields reliable, linear results with minimal autoluminescence background and superior sensitivity.

Firefly Luciferase Assay Kits (available in 150-assay and 1000-assay kits)

Kit Components:

• D- Luciferin, 10mg each
• Coelenterazine
• Passive Lysis Buffer
• Firefly Luciferase Assay Buffer
• Renilla Luciferase Assay Buffer
• Renilla Luciferase Enhancer

Features:
Linearity and Sensitivity
Linear correlation between luciferase gene and light output for transfection using 0.001 ug to 1 ug DNA of either Firefly or Renilla luciferase reporter constructs.
Low Autoluminescence
Reduced autoluminesence background for Renilla luciferase and increased sensitivity.
Convenience
One assay kit for both luciferase assays.

Description:
Firefly and Renilla luciferases are widely used as reporter genes for studying gene regulation and function, and for pharmaceutical screening. Renilla Luciferase is often used in conjunction with Firefly Luciferase as a normalizing transfection control or for multiplex transcriptional reporter assays. As with many enzymes, Firefly luciferase and Renilla luciferase follow Michaelis-Menten kinetics and thus maximum light output is not achieved until substrates (above the Km) and co-factor are present in large excess. When assayed under these conditions, light emitted from the reaction is directly proportional to the number of luciferase enzyme molecules. MIP's Firefly & Renilla luciferase assay kit is designed for detection and quantification of Firefly and Renilla luciferase reporter enzymes from cultured cells in a simple, efficient and linear fashion.

D-Luciferin, Potassium Salt 1g

• Storage: Store at -20°C
• Protect from light

D-Luciferin is a natural compound isolated from fireflies and is a substrate for the enzyme luciferase. Chemiluminescence at 560 nm is generated when luciferin is oxidized by an ATP-dependent process, catalyzed by luciferase. Luciferase is encoded by the luc gene, which is widely used as a reporter gene in a variety of cells. Because of the intrinsic low background of chemiluminescence technique, detection of the luc gene expression can be made at very low level. In addition, luciferin/luciferase has been used to measure 10 -15 molar quantity of ATP. White solid soluble in DMSO and slightly soluble in water.